Fast Protein and Metabolites (Nucleotides and Nucleosides) Liquid Chromatography Technique and Chemical Sensor for the Assessment of Fish and Meat Freshness

Fast protein and metabolite liquid chromatography (FPLMC) was introduced years ago to enable the easy separation of high-molecular compounds such as proteins from small molecules identification low-molecular substances. In this paper, method is applied for rapid evaluation freshness monitoring aging animal meat fish. A novel chromatographic sensor developed with a deep UV LED-based photometric detection unit (255–265 nm), an original flow cuvette registration scheme; processing chromatogram takes approximately 15 min. Strict isochronism between elution ATP metabolites, mainly hypoxanthine (Hx) inosine monophosphate (IMP), time maturation or fish, discovered. new index H* = [Hx]/[IMP] introduced, which proportional instrumental delay in FPMLC chromatograms: < 0.5 indicates presence (IMP) high quality Reasonably strong correlations were revealed data obtained by total volatile basic nitrogen TVB-N (for fish) fatty acids VFA meat) content. Moreover, putative nucleotide salvage increase concentration IMP observed fish after heat treatment using NMR technique.